In the line of code that is being used to read the image, you are concatenating the filename (a string/character array) with a 64x3 double matrix
[temp cmap]=imread([temp cmap]);
This may be the result of a copy and paste error, so you should try the following instead
filename = strcat(file_path, '\','0000',num2str(i-1),'.tif');
[img,imgCmap] = imread(filename);
I've changed some of the variable names to make them either give a better idea of what they represent, and/or so as not to conflict with previously defined variables (like the gray colour map).
Note that there is a problem with the F assignment. Remember that MATLAB indexing starts at 1, so you do not want to subtract one when choosing the index of F to update (especially as i starts at one). The line of code
F(i-1)=im2frame(temp,cmap);
should change to
F(i)=im2frame(img,imgCmap);
You could simplify your code by using sprintf instead of strcat and pad automatically the number in the file name with the appropriate number of zeros. Try replacing your above two loops with the following
for k=1:24
filename = fullfile(file_path,sprintf('%04d.tif',k-1));
[img,imgCmap] = imread(filename);
F(k) = im2frame(img,imgCmap);
end
fullfile to combine the path and name of the file. (I replaced the index i with k since i and j are also used to represent the imaginary number.)
